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Image Search Results
Journal: Endocrinology
Article Title: Estrogen receptor-α variant, ER-α36, is involved in tamoxifen resistance and estrogen hypersensitivity.
doi: 10.1210/en.2013-1116
Figure Lengend Snippet: Figure 2. TAM-resistant ER-positive breast cancer MCF7 cells express high concentrations of endogenous ER-36. (A) ER-positive breast cancer MCF7 cells and TAM-resistant MCF7 cells (MCF7/TAM) cells were treated with indicated concentrations of TAM for 7 days, and surviving cells were counted. The columns represent the means of 3 experiments; bars, SE. *P .05 for MCF/TAM cells treated with vehicle vs cells treated with 1M TAM. #P .01 for MCF/TAM cells vs MCF cells treated with indicated concentrations of TAM. (B) Western blot analysis of the expression concentrations of ER-36 and ER-66, EGFR, and HER2 in MCF7 and MCF7/TAM cells.
Article Snippet: Anti-phospho-p44/42 ERK (Thr202/Tyr204) (197G2) mouse monoclonal antibody (mAb), anti-p44/42 ERK (137F5) rabbit mAb, anti-phospho-AKT (Ser473) (D9E) rabbit mAb and anti-AKT (pan) (C67E7) rabbit mAb, anti
Techniques: Western Blot, Expressing
Journal: Endocrinology
Article Title: Estrogen receptor-α variant, ER-α36, is involved in tamoxifen resistance and estrogen hypersensitivity.
doi: 10.1210/en.2013-1116
Figure Lengend Snippet: Figure 3. ER-36 is involved in TAM resistance. (A) Western blot analysis of the concentrations of ER-36 and ER-66, EGFR, and HER2 proteins in MCF7 cells, MCF7/TAM cells transfected with the empty expression vector (MCF/TAM/Vector), and MCF7/TAM cells transfected with an ER-36-specific shRNA expression vector (MCF7/TAM/Si36). (B) Cells were treated with indicated concentrations of TAM for 7 days, and the numbers of surviving cells were determined. The columns represent the means of 3 experiments; bars, SE. *P .05 for MCF/ TAM/Vector cells treated with vehicle vs cells treated with 1M TAM.
Article Snippet: Anti-phospho-p44/42 ERK (Thr202/Tyr204) (197G2) mouse monoclonal antibody (mAb), anti-p44/42 ERK (137F5) rabbit mAb, anti-phospho-AKT (Ser473) (D9E) rabbit mAb and anti-AKT (pan) (C67E7) rabbit mAb, anti
Techniques: Western Blot, Transfection, Expressing, Plasmid Preparation, shRNA
Journal: Endocrinology
Article Title: Estrogen receptor-α variant, ER-α36, is involved in tamoxifen resistance and estrogen hypersensitivity.
doi: 10.1210/en.2013-1116
Figure Lengend Snippet: Figure 4. ER-positive breast cancer cells with elevated concentrations of ER-36 protein are resistant to TAM. (A) Western blot analysis of the lysates from ER-positive breast cancer MCF7 cells, MCF7 cells with forced expression of ER-36 (MCF7/ER36), and H3396 cells with high concentrations of endogenous ER-36 protein. The concentrations of EGFR and HER2 proteins are also shown. (B) Cells were treated with indicated concentrations of TAM for 7 days, and the numbers of surviving cells were counted. The columns represent the means of 3 experiments; bars, SE.
Article Snippet: Anti-phospho-p44/42 ERK (Thr202/Tyr204) (197G2) mouse monoclonal antibody (mAb), anti-p44/42 ERK (137F5) rabbit mAb, anti-phospho-AKT (Ser473) (D9E) rabbit mAb and anti-AKT (pan) (C67E7) rabbit mAb, anti
Techniques: Western Blot, Expressing